Denture-related stomatitis is probably the most common form of oral candidiasis and its reported prevalence varies widely ranging up to 65%. In this in vitro study, we evaluate the effect of delmopinol application on Candida albicans adherence on heat-cured acrylic resin, cold-cured acrylic resin and microwave-cured acrylic resin.

A total of 40 specimens of each type of acrylic resin were made; 20 specimens of each type were contaminated before delmopinol treatment and 20 specimens were contaminated after delmopinol treatment. The each specimen in each tube was individually transferred to a spectrophotometer at 530 nm wavelength in order to measure the turbidity degree, through the transmittance. Aliquots of 10 μl of each tube was then collected and inoculated into agar Sabouraud plates containing 500 mmol/l of sucrose, which was incubated for 24 hours at 37oC, in order to check microbial growth. Two-way ANOVA analysis of variance test and posthoc Turkey's test were carried out to ascertain the level of significance (p < 0.001) of various observations.

Mean of the turbidity degree of contamination after delmopinol application for heat-cured acrylic resin specimens (A1) 1440.80, colony forming units (CFU) (⨯10⁶/ml), cold-cured acrylic resin specimens (B1) 833.30 CFU (⨯10⁶/ml) and microwave-cured acrylic resin specimens (C1) 944.70 CFU (⨯10⁶/ml) was significantly higher than the mean of the turbidity degree of contamination before delmopinol application for heatcured acrylic resin specimens (A2) 550.85 CFU (⨯10⁶/ml), coldcured acrylic resin specimens (B2) 341.45 CFU (⨯10⁶/ml) and microwave-cured acrylic resin specimens (C2) 451.50 CFU (⨯10⁶/ml).

In case of contamination after delmopinol application, heat-cured acrylic resin showed maximum reduction in adherence of C. albicans in the study.

Shakya P, Jain D, Rahangdale T. Evaluation and Comparison of Effect of Delmopinol Application on Adherence of Candida albicans on Denture Fitting Surface on Three Types of Acrylic Resin: An in vitro Study. Int J Prosthodont Restor Dent 2012;2(4):129-135.

To evaluate the hemocompatibility of titanium after various surface treatments.

A total of 27 disk-shaped specimens (3 ⨯ 10.0 mm) were prepared from a cylindrical rod of medical grade titanium. The disks were divided into three groups, of which one was considered as the control (mechanical surface polished surface). The other groups being sandblasted disks and anodized disks. Surface evaluation was done for sandblasted and anodized disks with scanning electron microscope. The specimens were placed in polystyrene culture plates and agitated with phosphate buffered saline for 5 minutes before they were exposed to blood taken from human volunteer. The materials were under 30 minutes agitation at 75 ± 5 rpm using an Environ shaker thermostated at 35 ± 20°C. The total hemoglobin from the initial sample was measured using automatic hematology analyzer. Percentage hemolysis, thrombin time, platelet adhesion and activation were assessed. Significant differences between different treated titanium materials were determined using Minitab® Version 15.1.1.0. A two-sample t-test was performed to find the p-values for different groups of data.

After 30 minutes of agitation, cells began to spread on the test surfaces. There was a clear reduction in the number of platelets before and after exposure to titanium samples. Reduction of leukocytes was seen to a least extent on the anodized surface. Rough surface induced higher hemolysis than other groups. Platelet reduction and leukocyte reduction in all the three surfaces was quite higher than that obtained for reference plate. Surface variation has no significance on the thrombogenic capabilities of medical grade titanium (p < 0.05).

The hemocompatibility of medical grade titanium did not vary with different surface modifications.

BhavanChand Y, Ranzani R, Annapoorani H. Evaluation of Hemocompatibility of Titanium after Various Surface Treatments: An in vitro Study. Int J Prosthodont Restor Dent 2012;2(4):136-142.

Tripathi S, Singh RD, Singh SV, Arya D. A Simplified Approach of Customized Acrylic Nasal Stent Fabrication. Int J Prosthodont Restor Dent 2012;2(4):143-145.

Surgical removal of tumors in the mandible leads to discontinuity of bone which requires surgical reconstruction of the defect to provide a suitable tissue foundation for an acceptable prosthesis. At times when reconstructive surgeries are not possible, prosthetic rehabilitation is a successful alternative. In the present article, prosthetic rehabilitation using neutral zone technique has been discussed in the patient of marginal mandibulectomy.

The fabrication procedure of the removable partial denture was modified to achieve adequate retention and stability in a 22-year-old female patient with the anterior mandibulectomy. Polished surface contours and tooth positioning were recorded with modified neutral zone technique and mandibular teeth were characterized to provide esthetics.

The modified neutral zone technique enhanced the stability of the partial denture. A marked improvement in esthetics, function and psychology was noticed.

Tripathi S, Singh V, Singh SV, Arya D. Modified Neutral Zone Technique for the Partial Mandibulectomy Patient. Int J Prosthodont Restor Dent 2012;2(4):146-149.

Vaidya S, Khalikar A, Dange SP, Desai R. Complications and their Management in Implantology. Int J Prosthodont Restor Dent 2012;2(4):150-155.

Rajendran S, Baburajan. Combination Syndrome. Int J Prosthodont Restor Dent 2012;2(4):156-160.