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VOLUME 7 , ISSUE 2 ( April-June, 2017 ) > List of Articles

RESEARCH ARTICLE

Comparative Evaluation of Antimicrobial Efficacy of Neem, Miswak, Propolis, and Sodium Hypochlorite against Enterococcus faecalis using EndoVac

Priya Daga, Hemant Asrani, Shanin Farista, Praveen Mishra

Citation Information : Daga P, Asrani H, Farista S, Mishra P. Comparative Evaluation of Antimicrobial Efficacy of Neem, Miswak, Propolis, and Sodium Hypochlorite against Enterococcus faecalis using EndoVac. Int J Prosthodont Restor Dent 2017; 7 (2):60-65.

DOI: 10.5005/jp-journals-10019-1178

License: CC BY 3.0

Published Online: 01-10-2011

Copyright Statement:  Copyright © 2017; The Author(s).


Abstract

Aim

To compare the antimicrobial efficacy of herbal irrigants neem, miswak, propolis with sodium hypochlorite using conventional needle irrigation and EndoVac irrigation system against Enterococcus faecalis.

Materials and methods

A total of 120 extracted single-rooted mandibular premolar teeth were infected for 21 days with E. faecalis after instrumentation with ProTaper system. Before irrigation procedure, dentinal shavings were collected in 1 mL of sterile broth and incubated. The optical density of each broth was measured using digital colorimeter and initial readings were recorded. Samples were then divided into four groups of 30 teeth each – Group I: Sodium hypochlorite irrigation, group II: Neem irrigation, group III: Miswak irrigation, group IV: propolis irrigation. Each group was further divided into two subgroups – (a) EndoVac irrigation (b) conventional needle irrigation. After irrigation, dentinal shavings were collected and optical density recorded. The values were analyzed statistically with Student's t test and analysis of variance followed by Tukey's honest significant difference test; p-value < 0.05 was considered to be statistically significant.

Results

The postirrigation optical densities in all the groups were significantly lower than preirrigation values. Sodium hypochlorite demonstrated better antimicrobial efficacy followed by propolis, neem, and miswak. Differences in optical density values for all irrigants are higher in EndoVac (p < 0.0001) compared with needle (p = 0.0009) group, but it failed to reach statistical significance.

Conclusion

Sodium hypochlorite proved to be a better irrigant followed by propolis, neem, and miswak. EndoVac irrigation system was more effective for elimination of E. faecalis than needle irrigation group.

How to cite this article

Daga P, Asrani H, Farista S, Mishra P. Comparative Evaluation of Antimicrobial Efficacy of Neem, Miswak, Propolis, and Sodium Hypochlorite against Enterococcus faecalis using EndoVac. Int J Prosthodont Restor Dent 2017;7(2):60-65.


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  1. Comparative evaluation of antimicrobial activity of miswak, propolis, sodium hypochlorite and saline as root canal irrigants by microbial culturing and quantification in chronically exposed primary teeth. Germs 2011 Dec;1(1):12-21.
  2. Comparison of antimicrobial efficacy of propolis, Morinda citrifolia, Azadiracta indica, triphala, green tea polyphenols and 5.25% sodium hypochlorite against Enterococcus fecalis biofilm. Saudi Endo J 2014 Aug;4(3):122-127.
  3. Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. Oral Surg Oral Med Oral Pathol 1983 Mar;55(3):307-312.
  4. In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endo J 2001 Sep;34(6):424-428.
  5. Solution of pulp tissue by chemical agents. J Am Dent Assoc 1941 Feb;28(2):223-225.
  6. Evaluation of antimicrobial efficacy of herbal alternatives (triphala and green tea polyphenols), MTAD, and 5% sodium hypochlorite against Enterococcus faecalis biofilm on tooth substrate: an In vitro study. J Endod 2010 Jan;36(1):83-86.
  7. Influence of various herbal irrigants as a final rinse on the adherence of Enterococcus faecalis by fluorescence confocal laser scanning microscope. J Cons Dent 2013 Jul-Aug;16(4):352-355.
  8. Dentinal tubule disinfection with 2% chlorhexidine gel, propolis, Morinda citrifolia juice, 2% povidone iodine and calcium hydroxide. Int Endod J 2010 May;43(5):419-423.
  9. Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials propolis and aloe vera against Enterococcus faecalis: an ex vivo study. Dent Res J (Isfahan) 2014 Jul;11(4):469-474.
  10. Comparative evaluation of propolis, metronidazole with chlorhexidine, calcium hydroxide and Curcuma longa extract as intracanal medicament against E. faecalis – an in vitro study. J Clin Diag Res 2015 Nov;9(11):ZC19-ZC21.
  11. Comparison of the EndoVac system to needle irrigation of root canals. J Endod 2007 May;33(5):611-615.
  12. Antimicrobial efficacy of two irrigation techniques in tapered and nontapered canal preparations: an in vitro study. J Endod 2008 Nov;34(11):1374-1377.
  13. Comparative safety of various intracanal irrigation systems. J Endod 2009 Apr;35(4):545-549.
  14. Ex vivo antimicrobial efficacy of the Endovac® system plus photodynamic therapy associated with calcium hydroxide against intracanal Enterococcus faecalis. Int Endod J 2012 Nov;46(6):499-505.
  15. Enterococcus faecalis: Its role in root canal treatment failure and current concepts in retreatment. J Endod 2006 Feb;32(2):93-98.
  16. Efficacy of various concentrations of NaOCl and instrumentation techniques in reducing Enterococcus faecalis within root canals and dentinal tubules. Int Endo J 2006 Jan;39(1):10-17.
  17. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod 2000 Jun;26(6):331-334.
  18. Enterococcus faecalis biofilms eradication by root canal irrigants. J Endod 2009 May;35(5):711-714.
  19. Efficacy of passive ultrasonic irrigation with natural irrigants (MCJ, aloe-vera, and propolis) in comparison with 1% NaOCl for removal of E. faecalis biofilm: an in-vitro study. Indian J Dent Res 2013 Jan-Feb;24(1):35-41.
  20. An in vitro evaluation of antimicrobial activity of five herbal extracts and comparison of their activity with 2.5% sodium hypochlorite against Enterococcus faecalis. Indian J Dent Res 2015 Dec;26(5):524-527.
  21. Antibacterial, antifungal and antiviral activity of propolis of different geographical origin. J Ethanopharmacol 1996 Mar;64(3):235-240.
  22. Comparative evaluation of antimicrobial activity of neem, propolis, turmeric, liquorice and sodium hypochlorite as root canal irrigants against E. faecalis and C. albicans – an in vitro study. Endod J 2013 Dec;25(2):38-45.
  23. Comparison of antimicrobial activities of Moringa oleifera leaf, propolis, 2% chlorhexidine gluconate and MTAD on E. faecalis – an in-vitro study. Res J Pharm Bio Chem Sci 2014 May-Jun;5(3):163-173.
  24. Influence of irrigation regimens on the adherence of Enterococcus faecalis to root canal dentin. J Endod 2008 Jul;34(7):850-854.
  25. Evaluation of antimicrobial efficacy of four medicinal plants extracts used as root canal irrigant on Enterococcus faecalis: an in-vitro study. Int Dent Med J Adv Res 2015 Dec;1:1-8.
  26. Comparison of the antibacterial efficiency of neem leaf extract and 2% sodium hypochlorite against E. faecalis, C. albicans and mixed culture – an in vitro study. Endodontology 2010;22(1):8-12.
  27. Comparison of the antibacterial efficiency of neem leaf extracts, grape seed extracts and 3% sodium hypochlorite against E. faecalis – an in vitro study. J Int Oral Health 2013 Dec;5(6):61-66.
  28. Comparative evaluation of antimicrobial activity of herbal vs chemical root canal irrigants against E. faecalis – an in vitro study. Int J Adv Res 2015 Sep;3(9):1563-1572.
  29. Comparison of the effectiveness of three irrigation techniques in reducing intracanal Enterococcus faecalis populations: an in vitro study. J Endod 2009 Oct;35(10):1422-1427.
  30. Comparison of the antimicrobial efficacy of irrigation using the EndoVac to endodontic needle delivery. J Endod 2010 Mar;36(3):509-511.
  31. Comparison of regrowth of Enterococcus faecalis in dentinal tubules after sealing with gutta-percha or Resilon. J Endod 2008 Apr;34(4):445-448.
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