Purpose: To evaluate and compare the antifungal activity of four different essential oils incorporated in tissue conditioner on Candida albicans. Materials and methods: A total of 45 tissue conditioner (Viscogel) samples were fabricated either with different oils or without oils, forming five groups (n = 9/group). A standard amount of 2.2 gm of polymer to 1.8 gm of liquid was used. Liquid monomer without oils (control group) and 20% concentration of four oils [tea tree (Melaleuca alternifolia group), clove (Syzygium aromaticum group), coriander (Coriandrum sativum group), pista (Pistacia vera group)] were homogeneously mixed with the powder to formulate the autopolymerized tissue conditioner samples and stored in distilled water for 7 days. The antifungal properties of the different tissue conditioner samples were assessed by the agar punch well method after 24 hours, and the minimum inhibition zone (MIZ) was measured every day until 7 days. The statistical analysis was done using a one-way analysis of variance (ANOVA), followed by a post hoc test. Results: The tissue conditioner with clove oil (S. aromaticum group) had the highest minimum inhibition diameter (21 mm), followed by pista oil (P. vera group; 20.8 mm), tea tree oil (M. alternifolia group; 20.6 mm), coriander oil (C. sativum group; 20.5 mm), and the control group (20 mm). The difference was statistically significant (p = 0.001). Post hoc analysis showed a significant difference (p = 0.001) between the control group and the four tested groups in minimum inhibition diameter. The intergroup comparison of tested groups showed no significant difference. Conclusion: The antifungal activity of Viscogel with different oils used in the study was superior compared to the control group. The antifungal activity of Viscogel with clove oil (S. aromaticum) against C. albicans was greater compared to other oils used. Within the limitations of this study, the use of these oils along with Viscogel seems beneficial in inhibiting fungal growth.